Premium
Application of ELISA and ELADA Assay (Double Substrate Immunoassay) to Red Blood Cell Antigens
Author(s) -
Makler M. T.,
Pesce A. J.,
Campbell J. A.,
Green T. R.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21381201802.x
Subject(s) - antigen , antiserum , immunoassay , antibody , titer , red blood cell , microbiology and biotechnology , chemistry , biology , immunology , biochemistry
A double substrate immunoassay has been developed that allows simultaneous quantitation and localization of red blood cell surface antigens. The assay can be used to quantitate surface antigens, or the titer of antisera specific to cell‐surface antigens, on the red blood cell. The assay has been adapted to the study of both D antigen and erythrocyte membrane (EM) antigen, which were found to be uniformly dispersed over the surface of the cell. The number of EM antigens per cell appeared to be approximately twenty times that of the D antigen. For typing antisera, this assay makes it feasible to express antibodies in absolute units (μg antibody) rather than conventional dilution titers, and thus offers a simpler means of rapidly comparing antisera.