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Neutrophil Migration in Leukopenic Dogs: In Vivo Comparison of Granulocyte Function after Transfusion of Leukapheresis Concentrates or Purified Granulocytes Isolated by Counterflow Centrifugation‐Elutriation
Author(s) -
Jemionek J. F.,
Contreras T. J.,
Baker W. H.,
Stevens D. N.,
Bernhards F. W.,
Walden D. A.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21381201796.x
Subject(s) - leukapheresis , granulocyte , centrifugation , elutriation , in vivo , medicine , immunology , chemistry , biology , chromatography , microbiology and biotechnology , stem cell , organic chemistry , cd34
Granulocytes from dogs obtained by continuous‐flow centrifugation leukapheresis were purified by counterflow centrifugation‐elutriation using a modified rotor and enlarged separation chamber. The separation chamber has a threefold increase in volume capacity, as compared to the commercial Beckman Instruments' separation chamber, and the quantity and purity of the granulocytes recovered are suitable for purified granulocyte transfusion studies in a 10‐kg canine animal model. Transfusion of these cells into cyclophosphamide‐induced neutropenic animals permits an analysis of granulocyte chemotactic ability in terms of migration into skin chambers filled with endotoxin‐activated serum. The purified granulocyte function in vivo was compared to the activity of granulocytes present in leukapheresis concentrates. The data show that transfused granulocytes isolated by counterflow centrifugation‐ elutriation from leukapheresis concentrates retain identical in vivo chemotactic activity, as compared to granulocytes present in transfused leukapheresis concentrates.