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Blood Preservation 35
Author(s) -
Dawson R. B.,
Hershey R. T.,
Myers C. S.,
Miller R. M.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21281178161.x
Subject(s) - dihydroxyacetone phosphate , phosphate , ascorbic acid , preservative , chemistry , inorganic phosphate , food science , biochemistry
DHA (dihydroxyacetone, 60 mM) with ascorbic acid (d‐ascorbate, 10 mM) kept 2,3‐DPG concentrations above normal for six weeks. Levels of 2,3‐ DPG were below normal after four weeks with DHA alone and after two weeks with DHA‐ascorbate‐phosphate. As in previous studies, high phosphate concentrations decreased 2,3‐DPG maintenance. ATP maintenance was best achieved with the following (in order of performance): DHA‐ phosphate (20 mM); DHA‐phosphate (10 mM); the control, CPD‐adenine preservative; Phosphate 20 mM; and DHA. DHA with ascorbate provides normal 2,3‐DPG for six weeks. The adverse effects of DHA and DHA with ascorbate on ATP levels are modified by 10 mM phosphate.