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The Rapid Identification of Chido and Rodgers Antibodies Using C4d‐ Coated Red Blood Cells
Author(s) -
Judd W. J.,
Kraemer K.,
Moulds J. J.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21281178155.x
Subject(s) - antibody , identification (biology) , medicine , virology , immunology , biology , botany
A simple method for the recognition of anti‐Ch and anti‐Rg, using red blood cells coated in vitro with C4d, is described. Of 58 anti‐Ch and 20 anti‐Rg sera studied, all caused direct agglutination of C4d‐coated red blood cells in tests incubated at room temperature. Moreover, selected sera were shown to react strongly (3+ to 4+) with C4d‐coated red blood cells when tested by the indirect antiglobulin test without prior incubation at 37 C. Sera containing other high‐titer, low avidity (HTLA) antibodies were either nonreactive with C4d‐coated red blood cells, or reacted to the same degree with “non‐C4d‐coated” trypsin‐ treated red blood cells. Similar findings were obtained in tests with non‐HTLA antibodies of assorted specificities, including a variety of alloantibodies to high‐incidence red blood cell antigens. These findings indicate that the use of C4d‐coated red blood cells in investigative immunohematology may aid in the rapid identification of Ch and Rg antibodies.