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A Trypsin Method for Coating Human Red Blood Cells with Plasma C3 and/or C4
Author(s) -
Hsu T. C. S.,
Steinberg J.,
Borley R. J.,
Sawitsky A.
Publication year - 1980
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1980.20480260285.x
Subject(s) - autoanalyzer , trypsin , chromatography , chemistry , reagent , biochemistry , enzyme
Simple procedures are described for the production of human red blood cells coated with C3 and/or C4 by brief trypsin treatment of cell‐plasma mixtures at room temperature. These cells (‘EC3/4’‐try) were unusual in that their agglutinability by anticomplement sera, as compared to sucrose‐water RBC, was similar when tested by AutoAnalyzer but was markedly reduced in manual testing, especially against anti‐C4. In spite of this, both ‘EC34’‐try and ‘EC3’‐try were found to be suitable indicator cells for determining the adequacy of anti‐C3c and/or anti‐C3d activities in reagents used for manual antiglobulin tests.