Premium
In Vitro Evaluation of Platelets Stored in CPD‐Adenine Formulations
Author(s) -
Bolin R. B.,
Cheney B. A.,
Simpliciano O. A.,
Peck C. C.
Publication year - 1980
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1980.20480260272.x
Subject(s) - platelet , in vitro , adenosine triphosphate , adenosine diphosphate , chemistry , preservative , adenine nucleotide , biochemistry , andrology , nucleotide , food science , platelet aggregation , medicine , gene
Little information is available about the effect of adenine and added glucose on stored platelets. Two new formulations, CPDA‐2 and CPDA‐3, contain 34 mg adenine per 63 ml preservative and extra glucose (1.75 and 2.0 times the glucose in standard CPD). We have studied the in vitro integrity of platelet concentrates stored in CPD, CPDA‐I, CPDA‐2, and CPDA‐3 at 22 C for 72 hours. Morphology score, pH, platelet size, population distribution parameters, and electron microscopic ultrastructure did not show any adverse effects which could be ascribed to the presence of adenine or extra glucose or both. No differences in platelet adenosine triphosphate (ATP) concentration or plasma glucose utilization during storage were found between CPD and CPDA‐I platelets. The results suggest that adenine and added glucose in these preservatives are not detrimental to platelets in vitro by the measures employed.