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Blood preservation. XLIII. Studies on the ascorbate mechanisms of maintaining red cell 2,3‐DPG
Author(s) -
Dawson RB,
Dabezies M,
Hershey RT,
Myers CS,
Miller RM
Publication year - 1980
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1980.20380214898.x
Subject(s) - glutathione , chemistry , red cell , red blood cell , preservative , ascorbic acid , diphosphoglycerate , biochemistry , blood cell , medicine , food science , enzyme , hemoglobin
Our previous experiments on the mechanisms of ascorbate's effect on the red blood cell failed to show an effect of iodoacetate (IA), a sulfydryl inhibitor. In this study, in contrast to the previous, iodoacetate (85 micromolar) was seen to prevent continued red blood cell metabolism. During the first weeks there was an absence of a continual fall in pH; ATP levels were depressed below half normal; and 2,3‐DPG levels fell to very low values within the first week. ATP was best maintained in the control preservative and next best maintained, at adequate levels, with ascorbate, 5 mM, with and without glutathione, 5 mM. 2,3‐DPG levels were well maintained with ascorbate and ascorbate with glutathione. Poor ATP maintenance and rapid decreases in 2,3‐DPG were observed with iodoacetate, IA plus ascorbate, and IA plus ascorbate and glutathione.