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The preparation of leukocyte‐poor red blood cells: a comparative study
Author(s) -
Meryman HT,
Bross J,
Lebovitz R
Publication year - 1980
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1980.20380214893.x
Subject(s) - red blood cell , saline , red cell , medicine , sedimentation , erythrocyte sedimentation rate , immunology , andrology , blood cell , surgery , biology , paleontology , sediment
Thirteen methods of prepararing leukocyte‐poor red blood cells were compared for their effectiveness in removing leukocytes. When the starting material was six to ten days old, the residual leukocyte count was three to four times lower than when fresh material was used and no granulocytes could be found. The most effective manual procedure was a single upright sedimentation of six‐ to ten‐day‐old saline‐diluted red cells. Inverted sedimentation was no more effective in leukocyte depletion than upright sedimentation. Automated cell processors were no more effective than several of the better manual methods. Freezing and deglycerolizing resulted in a ten‐fold improvement in leukocyte removal with less red blood cell loss but at higher cost than the nonfreezing methods. The study indicates that unless the minimal leukocyte count of frozen red blood cells is essential, a single upright sedimentation of saline‐diluted, six‐ to ten‐day‐old red blood cells is currently the most effective procedure for preparing leukocyte‐poor red blood cells.