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Antibody to Hepatitis B Surface Antigen: Immunochemical Purification from Standard Immune Serum Globulin
Author(s) -
Dodd R. Y.
Publication year - 1980
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1980.20280169963.x
Subject(s) - hbsag , antibody , antigen , chemistry , hepatitis b , hepatitis b virus , immune system , globulin , microbiology and biotechnology , virology , immunology , biology , virus
Hepatitis B surface antigen (HBsAg) may be insolu‐bilized onto a controlled pore glass (CPG) matrix by simple adsorption from acidified serum or plasma. The resultant complex has the properties of an HBsAg‐specific immunoadsorbent. In this study, CPG‐HBsAg complexes have been used to adsorb anti‐HBs from standard immune serum globulin. Specific antibodies were eluted with acidic or chaotropic buffers. Recoveries ranging from 29 to 46 per cent, with purifications of approximately 1000‐fold were obtained. Two procedures designed to inactivate hepatitis B virus had no effect upon the immunoadsorbent properties of the CPG‐HBsAg complex. The possibility of using this simple method to prepare hepatitis B immune globulin is discussed.