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Ultrastructure of Human Platelets Processed for Transfusion Under Standard Blood Bank Conditions
Author(s) -
Lee E. L.,
Azar H. A.,
Kasnic G.
Publication year - 1979
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1979.19680104099.x
Subject(s) - agitator , platelet , centrifugation , ultrastructure , arc (geometry) , blood bank , cold storage , medicine , platelet transfusion , chemistry , materials science , biomedical engineering , surgery , andrology , biology , pathology , chromatography , mathematics , geometry , mass transfer , medical emergency , horticulture
Platelets processed for transfusion are routinely subjected to centrifugation, mechanical agitation and storage at either room temperature or 4 C. To evaluate morphologic changes associated with standard blood bank conditions, platelet units were grouped into four categories: linear agitation at room temperature, agitation at a 90° arc with a Hemolater at room temperature, agitation at a 360° arc with a commercial platelet agitator, and refrigeration at 4 C without agitation. Cold (4 C) preservation resulted in a surprisingly good preservation of platelet ultrastructure, even at 72 hours of storage. By contrast, harsh linear agitation at room temperature brought about marked alterations in platelet ultrastructure beginning at 24 hours. The Hemolator agitator with 90° arc was associated with good preservation of platelet organelles even at 72 hours, whereas marked degenerative changes were observed at this time in platelets processed with a commercial agitator with a 360° arc. These results indicate that platelets stored at 4 C or processed at room temperature with an agitator with 90° arc show the best preservation of ultrastructure.