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Factors that Influence the Process of 51 Chromium Labeling of Human Granulocytes Isolated from Blood by Counterflow Centrifugation
Author(s) -
Kurtz S. R.,
Carciero R.,
Valeri C. R.
Publication year - 1979
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1979.19479250176.x
Subject(s) - centrifugation , incubation , chemistry , chromatography , differential centrifugation , granulocyte , saline , chromium , suspension (topology) , phosphate , incubation period , andrology , biochemistry , medicine , biology , endocrinology , mathematics , homotopy , pure mathematics , organic chemistry
The uptake of 51 chromium by human granulocytes isolated from blood by counterflow centrifugation is influenced by the composition of the suspension medium, the incubation period, the temperature and the amount of 51 chromium used. Counterflow centrifugation‐elutria‐tion isolated approximately 98 per cent of the granulocytes from the blood. Acid‐citrate‐dextrose (ACD) plasma, citrate‐phosphate‐dextrose (CPD) plasma and heparinized plasma as resuspension media produced similar labeling results, whereas phosphate‐buffered saline resulted in three times greater uptake in 51 chromium. The greatest uptake of 51 chromium (4%) was achieved after incubation at 37 C for one hour in autologous plasma, and granulocyte integrity and metabolic function in vitro were not adversely affected.