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A Factor VIII Concentrate of Intermediate Purity and Higher Potency
Author(s) -
Smith J. K.,
Evans D. R.,
Stone V.,
Snape T. J.
Publication year - 1979
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1979.19379204211.x
Subject(s) - potency , solubility , chromatography , chemistry , syringe , fractionation , filtration (mathematics) , yield (engineering) , whole blood , medicine , surgery , materials science , biochemistry , mathematics , in vitro , organic chemistry , metallurgy , statistics , psychiatry
A fractionation method has been modified to produce a factor VIII concentrate of intermediate purity and a potency of about 12 iu/ml, suitable for injection by syringe. The solubility, ease of filtration and purity of the concentrate have been improved by including a stage in which contaminants are precipitated at controlled temperature, pH and ionic strength. A summary of the first 50 production‐scale batches is presented. Plasma can be harvested from whole blood and frozen within eight hours of donation, or after overnight storage of whole blood, with no significant difference in overall yield.