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False Positive and False Negative Reactions in HLA B‐27 Antigen Testing
Author(s) -
Larsen A. E.
Publication year - 1979
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1979.19279160299.x
Subject(s) - false negative reactions , human leukocyte antigen , medicine , immunology , antigen
The recent commercial availability of HLA B‐27 antiserum now enables laboratories without prior experience in HLA typing to test for this antigen. False positive and false negative reactions frequently occur and require judicious interpretation. In our laboratory, 30 of 100 specimens were positive for B‐27 and 70 were negative. Of the 30 positives, 10 (33%) failed to react against every antiserum tested. Twenty‐nine of the 70 negative specimens (41%) reacted falsely with one or more B‐27 antisera. A significant cause of false positive reactions was cross‐reaction of the B‐7 antigen with B‐27 antisera (13 of 15 B‐7 positive, B‐27 negative specimens). For valid B‐27 testing, use of more than one B‐27 antiserum is indicated.