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A New Method of Antibody Elution From Red Blood Cells
Author(s) -
ChanShu S. A.,
Blair O.
Publication year - 1979
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1979.19279160290.x
Subject(s) - abo blood group system , elution , antibody , autoimmune hemolytic anemia , hemolytic disease of the newborn (abo) , immunology , medicine , red blood cell , red cell , chromatography , hemolysis , chemistry , biology , pregnancy , fetus , genetics
A method of antibody elution from red blood cells using xylene is described. It can be completed in 15 minutes after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin's ether method. It was as effective as Landsteiner and Miller's heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.