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HLA Antigens in Plasma Protein Fraction and Albumin
Author(s) -
Dickerson J. M.,
Perkins H. A.
Publication year - 1978
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1978.18478251246.x
Subject(s) - antiserum , antigen , albumin , human leukocyte antigen , immunology , serum albumin , medicine , antibody , dialysis , blood proteins , tissue typing , typing , chemistry , biology , genetics
Renal allograft survival is significantly worse than average in recipients who have never had blood transfusions. It has been speculated that this might be explained by the fact that patients on chronic dialysis often received plasma protein fraction (PPF) instead of blood and that HLA antigens from serum were likely to be present in PPF. We have examined four different lots of both PPF and albumin for the presence of HLA antigens. Using the microlymphocyto‐toxicity technique, each lot was tested for the ability to neutralize ten different antisera containing antibodies against well‐defined HLA antigens. In every case, no inhibition was observed—even when the ratio of blood derivative to antiserum was as high as 256:1. These results suggest that if PPF and albumin do contain HLA antigens, they are present at levels too low to be detected by the cytotoxicity test routinely used in HLA typing.