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Autoagglutination Dispersal Utilizing Sulphydryl Compounds
Author(s) -
Reid M. E.
Publication year - 1978
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1978.18378205146.x
Subject(s) - incubation , red blood cell , red cell , chemistry , antigen , in vivo , incubation period , biochemistry , immunology , biology , medicine , microbiology and biotechnology
Autoagglutination caused by powerful IgM cold autoagglutinins can be dispersed by sulphydryl compounds to provide an unagglutinated red blood cell suspension suitable for immunohematologic testing. For this 0.1 M 2‐mercaptoethanol is added to twice washed red blood cells. Following agitation for 10 minutes at 37 C an unagglutinated red blood cell suspension is obtained without adverse effect to red blood cell antigens and without removal of in vivo bound IgG or complement. Dithiothreital may be substituted for 2‐mercaptoethanol if the incubation time is increased to 15 minutes.