Premium
Variation in Gamma Counter Efficiency Used in Radioimmunoassay Testing for Hepatitis B Surface Antigen and Antibody
Author(s) -
Bales Z. B.,
Patterson J. F.,
Hoofnagle J. H.,
Seeff L. B.
Publication year - 1978
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1978.18178118574.x
Subject(s) - radioimmunoassay , serial dilution , antibody , antigen , chromatography , medicine , chemistry , immunology , pathology , alternative medicine
Occasional discordant results on testing samples by radioimmunoassay for hepatitis B surface antigen and antibody led to this analysis of gamma counters used by different hospitals and testing laboratories. Eight dilutions of purified 125 I were sent under code to 11 institutions doing radioimmunoassay testing. Results demonstrated a wide range in background counts‐per‐minute (cpm) as well as considerable variation between gamma counters in per cent counting efficiency for 125 I (ranging from 20 to 48%.) These data support the need for regular monitoring and calibration of gamma counters to ensure low background cpm as well as maximum counting efficiency. This will give reproducible and reliable results in radioimmunoassay testing.