Premium
Large Scale Preparation of Leukocyte Concentrates and Further Purification of Lymphocytes and Granulocytes with the IBM 2991 Cell Processor
Author(s) -
Rivière A. Brutel,
VerhoefKarssen P. R.,
Wit J. J. F. M.,
Loos J. A.,
Prins H. K.
Publication year - 1977
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1977.17578014593.x
Subject(s) - buffy coat , centrifugation , ficoll , peripheral blood mononuclear cell , platelet , yield (engineering) , immunology , abo blood group system , chemistry , andrology , chromatography , medicine , biochemistry , in vitro , materials science , metallurgy
A procedure is described for the large‐scale production of pure suspensions of human lymphocytes and granulocytes. Platelet‐poor buffy‐coats, obtained by centrifugation of six units of ABO‐compatible ACD blood, were pooled and centrifuged again, resulting in a crude leukocyte concentrate containing approximately 60 per cent of the original amount of leukocytes. Further fractionation of such crude leukocyte concentrates was achieved by scaling up Bøyum's Ficoll‐Isopaque density gradient technique with the aid of an IBM 2991 blood cell processor. In this way 2.9 + 0.3 times 10 9 mononuclear leukocytes, consisting of 90 + 0.7 per cent lymphocytes and 5.3 + 0.3 times 10 9 polymorphonuclear leukocytes of 92 + 0.7 per cent purity could be isolated in one run. The overall yield was 36 per cent of the original amount of leukocytes.