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Ultrafiltrable Adenosine Triphosphate and 2,3‐Diphosphoglycerate Concentrations in Cold‐stored Human Erythrocytes
Author(s) -
Marshall W. E.,
Rassaian N.,
Greenwald L. S.,
Omachi A.
Publication year - 1977
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1977.17578014582.x
Subject(s) - diphosphoglycerate , adenosine triphosphate , tonicity , chemistry , metabolism , hemoglobin , red blood cell , biochemistry
Free ATP and DPG concentrations in ACD‐preserved human erythrocytes (rbc) were estimated by measuring these organic phosphates in ultrafiltrates of cell lysates prepared at 0 to 2 C. The free ATP concentration in hypotonic hemolysates decreased from 0.59 to 0.08 μmol/ml rbc during four weeks of cold storage. In French Pressure Cell lysates, free ATP increased from 0.23 to 0.47 μmol/ml rbc in the first week and then declined to 0.12 μmol/rbc during the following three weeks. In two weeks, the free DPG level decreased from 0.64 to 0.41 μmol/ml rbc in hypotonic lysates and from 1.08 to 0.15 μmol/ml rbc in French Pressure Cell lysates. It is proposed that, as total DPG concentration decreases during cold storage, free ATP concentration also decreases due to increased ATP binding to hemoglobin sites vacated by DPG. It is suggested that it is the change in the free rather than the total ATP which may be more relevant to the metabolism of the cold‐stored erythrocyte.