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Formation of Oxyadenine Metabolites in the Rabbit after Intravenous Administration of Adenine
Author(s) -
Bartlett G. R.
Publication year - 1977
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1977.17477216864.x
Subject(s) - urine , excretion , chemistry , chromatography , biochemistry
Adenine, 8‐oxyadenine, and 2,8‐dioxyadenine were analyzed, by Dowex‐50 column chromatography, in rabbit urine four hours after IV infusion of the following doses of adenine in mg/kg: 2, 4, 8.75, 17.5, 35, 70, 140 and 210. The average excretion in mole percentage of the dose was: adenine, 11.1; 8‐oxyadenine, 6.1; and 2,8‐dioxyadenine, 6.2. The percentage of excretion of 8‐oxyadenine and of 2,8‐dioxyadenine was unrelated to the dose of adenine. At doses of adenine of 35 mg/kg and above more than 80 per cent of the 2,8‐dioxyadenine in the urine was in the form of microscopic pale yellow spherical particles. The particulate 2,8‐dioxyadenine was found in the kidney in a very small amount at the dose of 70 mg/kg and in much greater quantities at 140 and 210 mg/kg.