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Blood storage XXIII: 2,3‐DPG maintenance for six weeks in a CPD‐adenine‐ inosine preservative with and without methylene blue
Author(s) -
Dawson R. B.
Publication year - 1977
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1977.17377196358.x
Subject(s) - inosine , preservative , methylene blue , chemistry , chromatography , medicine , food science , biochemistry , adenosine , photocatalysis , catalysis
In a pilot study the optimal concentration of inosine for 2,3‐DPG maintenance in a CPD‐adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3‐DPG maintenance was nearly normal for six weeks of storage in CPD‐adenine‐inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3‐DPG concentrations after the 3rd week. Finally, 2,3‐DPG levels were significantly better maintained in CPD‐adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(‐6)M), compared to CPD‐adenine‐inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3‐DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3‐DPG for prolonged (five to six weeks) liquid storage.