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Preferential Decrease in Thymus Dependent Lymphocytes during Storage at 4 C in Anticoagulant
Author(s) -
Grunow J. E.,
Lubet R. A.,
Ferguson M. J.,
Gaulden M. E.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1976.16677060244.x
Subject(s) - ficoll , whole blood , chemistry , anticoagulant , andrology , immunology , medicine , microbiology and biotechnology , biology , biochemistry , in vitro , peripheral blood mononuclear cell
Human lymphocytes stored at 4 C either as leukocyte concentrates (LCs) in citrate‐phosphate‐dextrose (CPD) or as whole blood anticoagulated with CPD show a rapid and marked decrease in the relative and absolute numbers of thymus derived (T) lymphocytes. Determinations were made on cells recoverable on a Ficoll‐Hypaque (F‐H) gradient. In evacuated LCs, the relative percentage of T cells dropped to less than 10 per cent within 72 hours with a concomitant increase in the relative percentage of bone marrow derived (B) cells to 80 per cent or more. LCs opened to the air and subsequently stored at 4 C displayed an even more precipitous decline in the relative percentage of T cells, reaching a 10 per cent level within 72 hours. The relative percentage of T cells in CPD‐anticoagulated whole blood samples stored at 4 C displayed similar decreases, reaching 20 per cent levels within 24 hours. The change in the relative percentage of T cells at the Ficoll‐Hypaque interface was shown to reflect a decrease in the total numbers of T cells placed on the F‐H gradient with time, since determinations of T and B cell numbers in NH 4 CI‐treated whole blood showed a 65 to 80 per cent decrease in the numbers of T cells within 24 hours in anticoagulated whole blood held at 4 C. Thus, it may be inferred that the T cell decrease is mediated via some interaction of anticoagulant, storage time, and some component(s) present in both LCs and whole blood.