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Plateletpheresis Residues: A Source of Large Quantities of Human Blood Lymphocytes
Author(s) -
Segel G. B.,
Lichtman M. A.,
Gordon B. R.,
MacPherson J. L.,
Nusbacher J.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1976.16577039302.x
Subject(s) - plateletpheresis , peripheral blood mononuclear cell , human blood , residue (chemistry) , peripheral blood , monocyte , immunology , lymphocyte , medicine , chemistry , biochemistry , platelet , apheresis , physiology , in vitro
The residue from single‐donor plateletpheresis contains a large number of human mononuclear cells. We have been able to harvest more than 1 × 10 9 viable lymphocytes for laboratory study from the leukocyte‐rich sediment that previously had been discarded. Prior to removal by adherence 5 × 10 8 monocytes were also available for further purification, if desired. The physical properties and response to phytohemagglutinin were very similar when lymphocytes isolated from plateletpheresis residues were compared with those obtained directly from venous blood.