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Use of a Low‐Ionic‐Strength Medium in Manual Tests for Antibody Detection
Author(s) -
Moore H. C.,
Mollison P. L.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1976.16476247048.x
Subject(s) - ionic strength , incubation , antibody , incubation period , chemistry , chromatography , albumin , serum albumin , immunology , medicine , biochemistry , aqueous solution
The present observations confirm the value of suspending red blood cells in a low‐ionic‐strength medium in the first stage of the indirect antiglobulin test; that is, during the period of incubation with antibody‐containing serum. The main advantage of this procedure is to shorten the time of incubation. In this respect a low‐ionic‐strength medium appears to be superior to albumin as a suspending medium for the red blood cells. A further advantage is to increase the uptake of certain antibodies; this effect was pronounced with selected Rh antibodies believed to be of low affinity.