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The Fate of Bacteria in Frozen Red Cells
Author(s) -
Kahn R. A.,
Meryman H. T.,
Syring R. L.,
Flinton L. J.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1976.16376225491.x
Subject(s) - enterobacter cloacae , acinetobacter calcoaceticus , enterobacter aerogenes , microbiology and biotechnology , bacteria , staphylococcus epidermidis , enterobacter , streptococcus , sarcina , biology , micrococcus , acinetobacter , disinfectant , staphylococcus , food science , chemistry , escherichia coli , enterobacteriaceae , staphylococcus aureus , antibiotics , biochemistry , gene , genetics , organic chemistry
Units of blood were intentionally contaminated with suspensions of either Aerobacter aerogenes, Escherichia coli, Bacillus subtilis, Enterobacter cloacae, Pseudomonas aeruginosa, Sarcina lutea, Seratia marcesens, Staphylococcus epidermidis, Streptococcus faecalis, Paracolabacterum aerogenoides (Enterobacter hafriae), Mima polymorpha or Acinetobacter calcoaceticus. When inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization resulted in roughly a two log reduction in the number of bacteria. When inoculation was made with a final concentration of between 10 1 and 10 5 organisms per milliliter, immediately following deglycerolization or following washing without glycerolization and freezing, no increase in the number of bacteria was seen after 72 hours storage at 4 C. Three of the 12 organisms studied decreased in number during 72 hours of storage. These data suggest that the current 24‐hour limit on the post‐thaw storage of frozen red blood cells may be unnecessarily restrictive.