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Use of Hydroxyethyl Starch To Improve Granulocyte Collection in the Latham Blood Processor
Author(s) -
Huestis D. W.,
White R. F.,
Price M. J.,
Inman M.
Publication year - 1975
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1975.15676082230.x
Subject(s) - hydroxyethyl starch , leukapheresis , plateletpheresis , platelet , medicine , trisodium citrate , liter , blood donor , anticoagulant , apheresis , surgery , chemistry , anesthesia , biochemistry , immunology , biology , stem cell , cd34 , genetics
This report describes a practical, relatively inexpensive system of leukapheresis and plateletpheresis, utilizing the Haemonetics blood processor. The results of 236 phereses of normal donors are reported, with particular attention to an evaluation of five different anticoagulant mixtures: ACD, 2 per cent citrate in saline, and three different combinations of citrate and hydroxyethyl starch (HES). These mixtures were compared with respect to their effectiveness in the harvesting of granulocytes and platelets, respectively. The best harvest of granulocytes (3.5 × 10 9 cells per liter of blood processed) was with 6 per cent HES anticoagulated with trisodium citrate. All solutions gave about the same platelet yields (a mean of 1.6 × 10 9 per liter of blood processed). Because of its higher citrate content, ACD caused three times as many donor reactions as the other solutions. The use of HES thus permits this widely available plateletpheresis system to be used for leukapheresis with only minimal procedural modification.