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Viable Lymphocytes in Frozen Washed Blood
Author(s) -
Telischi M.,
Krmpotic E.,
Moss G.
Publication year - 1975
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1975.15576082225.x
Subject(s) - blood preservation , medicine , chemistry , immunology , andrology
Samples of glycerolized, frozen, packed cells, were washed by each of three systems and then were cultured for viable lymphocytes using the short‐term culture method. Of the 39 samples, 17 (43.6%) showed definite evidence of growth of lymphocytes. Of the 17, 11 (64.7%) were washed by Elutramatic, two (11.8%) by Haemonetics, and four (23.5%) by IBM. The 4 C prefreeze (two to five days) as well as the –80 C (21 to 40 days) storage times were the same for both positive and negative specimens. In all samples, clumps of distorted granulocytes with pyknotic nuclei were seen in addition to a number of well‐preserved mononucleated white cells. In the 17 specimens which showed growth, incorporation of 3 H‐thymidine was seen only in the PHA‐induced blast cells; typical mitotic figures were seen in some cultures. These observations demonstrate that lymphocytes remain viable in frozen blood stored at –80 C up to 40 days. The significant difference in removal of viable lymphocytes noted in specimens washed by different instruments requires further evaluation.