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Evaluation of Platelet Cryopreservation Techniques by Isolated Kidney Perfusion
Author(s) -
Raymond S. L.,
Pert J. H.,
Dodds W. J.
Publication year - 1975
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1975.15375160355.x
Subject(s) - cryopreservation , dimethyl sulfoxide , platelet , perfusion , kidney , andrology , medicine , chemistry , biomedical engineering , biology , immunology , microbiology and biotechnology , embryo , organic chemistry
The isolated perfused rabbit kidney, a model system used previously to assess platelet function, was adapted for evaluation of human platelet cryopreservation techniques. A new, simple, efficient device for controlling cooling rates before, during, and after freezing was used. Platelet concentrates frozen with 5 per cent dimethyl sulfoxide (DMSO) under different conditions were the most effective of those tried in maintaining the hemostatic function and vascular integrity of perfused kidneys. Our studies indicate that the isolated, perfused rabbit kidney can be used to evaluate platelet cryopreservation techniques and is potentially adaptable for studies of organ cryopreservation.