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In Vitro Metabolism of Packed Erythrocytes Stored in CPD‐Adenine
Author(s) -
Bensinger T. A.,
Metro J.,
Beutler E.
Publication year - 1975
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1975.15275122807.x
Subject(s) - adenosine triphosphate , in vitro , preservative , metabolism , adenine nucleotide , chemistry , adenosine , biochemistry , nucleotide , food science , gene
In vitro metabolism of erythrocytes packed at 70 and 90 per cent hematocrits and stored in various CPD‐adenine preservatives was studied. It was found that maintenance of acceptable levels of adenosine triphosphate (ATP) for the full 42 days of storage could be accomplished only if glucose levels were doubled from the standard 138.7 mM concentration to 277.5 mM level. If glucose levels were doubled, the amount of adenine could be decreased from 4.07 mM (0.50 mM final concentration) to 2.04 mM (0.25 mM final concentration) with maintenance of ATP at greater than 2.0 μmoles/g Hb. 2,3‐diphosphoglycerate concentrations were essentially absent by 21 days in the various media studied. Thus, in vitro levels of ATP appear to be maintained at acceptable levels in a CPD media modified to contain 2 × glucose and 2.04 mM adenine.