Premium
An Anti‐Neomycin Antibody Interfering with ABO Grouping and Antibody Screening
Author(s) -
Hysell J. K.,
Gray J. M.,
Hysell J. W.,
Keck M. L.
Publication year - 1975
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1975.15175103504.x
Subject(s) - neomycin , abo blood group system , antibody , agglutination (biology) , chemistry , kanamycin , saline , chromatography , microbiology and biotechnology , medicine , immunology , biology , biochemistry , antibiotics , endocrinology
A patient's serum agglutinated most commercial antibody screening and panel identification red blood cells; however, it did not agglutinate either the cells of ABO compatible donors or washed, saline suspended commercial cells. When suspended in a solution containing 0.1 mg/ml neomycin, all cells, including the patient's, were agglutinated by the patient's serum. Inhibition did not occur by preincubation of either the patient's serum or the test cells with 10 mg/ml neomycin. Serum preincubation with butirosin, kanamycin, or neamine inhibited immediate agglutination in the presence of neomycin. The reactive group is, therefore, included in the neamine fragment. Ultracentrifugation segregated the antibody in the 7S fraction. Following separation by column chromatography, only fractions containing IgA demonstrated antineomycin activity.