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Mycosporine‐Like Amino Acid Intercalibration Effort Using Replicate Samples
Author(s) -
Zimba P. V.,
Boue S.
Publication year - 2002
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.38.s1.11.x
Subject(s) - biology , analyte , cyanobacteria , porphyra , chromatography , replicate , sample preparation , algae , botany , food science , bacteria , chemistry , genetics , statistics , mathematics
The measurement of mycosporine‐like amino acids (MAAs) has several inherent problems, including the lack of commercial standards for identification and quantification. This symposium provided the opportunity to coordinate analyses with six laboratories actively involved in MAA research. Two samples were provided to each laboratory and included freeze‐dried nori ( Porphyra sp.) and a freeze‐dried (filtered) sample of the cyanobacteria Microcystis aeruginosa Kützing. Each laboratory provided extraction methodologies, chromatograms of the identified peaks, as well as estimates of the concentration of each analyte. All laboratories were able to identify major chromatographic components of the samples ( Porphyra : shinorine, porphyra, mycosporine 2‐glycine, asterina, palythine; Microcystis : shinorine, porphyra). Sequential cold (4 °C for 18 h) and hot (45 °C for 2 h) extractions of the same sample resulted in differing analyte recovery. Several currently unidentified compounds were observed in freshwater samples.