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ENHANCEMENT OF INTRACELLULAR SAXITOXIN ACCUMULATION BY LIDOCAINE HYDROCHLORIDE IN THE CYANOBACTERIUM CYLINDROSPERMOPSIS RACIBORSKII T3 (NOSTOCALES) 1
Author(s) -
Pomati Francesco,
Neilan Brett A.,
Suzuki Takahiro,
Manarolla Gianluca,
Rossetti Carlo
Publication year - 2003
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.2003.02122.x
Subject(s) - cylindrospermopsis raciborskii , lidocaine hydrochloride , saxitoxin , biology , lidocaine , intracellular , toxicity , cyanobacteria , toxin , pharmacology , chromatography , biochemistry , chemistry , bacteria , genetics , organic chemistry , neuroscience
The metabolic effect of three different concentrations of lidocaine hydrochloride (0.01, 0.1, and 1 μM) on growth and saxitoxin (STX) production of the freshwater cyanobacterium Cylindrospermopsis raciborskii (Wolosznska) T3 was analyzed. Lidocaine hydrochloride increased both the growth rate and the final growth yield in the toxic cyanobacterium, with a maximum of 25% and 18% for a 1‐μM dose, respectively. Moreover, C. raciborskii T3 samples harvested at the end of the growth phase and analyzed for STX content by HPLC showed an increase in STX intracellular concentration of 14.3% and 49.3% after exposure to 0.01 and 0.1 μM lidocaine hydrochloride, respectively, whereas 1 μM lidocaine hydrochloride resulted in a 114% incremental change in STX content. The time course of the 1‐μM lidocaine hydrochloride effect showed the highest rate of increase in mean STX intracellular concentration (298%) within the first 2 h after induction. The increase in STX content induced by lidocaine hydrochloride in C. raciborskii T3 was dependent on the concentration of Na + ions in the culture medium and alkaline pH. The results suggest a possible action of lidocaine hydrochloride on membrane ion fluxes and the hypothesis of a potential linkage between cyanobacterial homeostasis and STX regulation.