Premium
CLONAL PROPAGATION OF LAMINARIA DIGITATA (PHAEOPHYCEAE) SPOROPHYTES THROUGH A DIPLOID CELL‐FILAMENT SUSPENSION
Author(s) -
Asensi Aldo,
Gall Erwan Ar,
Marie Dominique,
Billot Claire,
Dion Patrick,
Kloareg Bernard
Publication year - 2001
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.2001.037003411.x
Subject(s) - biology , sporophyte , botany , subculture (biology) , laminaria , laminaria digitata , ploidy , protein filament , algae , genetics , gene
In vitro cultures of Laminaria digitata (Hudson) Lamouroux stipe medullary explants (Laminariales, Phaeophyceae) gave rise to friable morula‐like masses of pigmented cells. Upon subculture under continuous white light, they grew out as filaments, which tended to dissociate into spherical isolated cells, leading to the establishment of a suspended cell‐filament culture. Depending on culture conditions, this cell‐filament suspension underwent various forms of growth, including regeneration of morphologically normal sporophytes, and this developmental pathway was stimulated by blue light. Genotyping with nine polymorphic microsatellite markers indicated that the regenerated sporophytes and the cell‐filament suspension shared the same genotype. As assessed by flow cytometric analysis of isolated nuclei, the cell‐filament suspension exhibited a 2C nuclear DNA content, whereas the regenerated sporophytes displayed a 4C level. Chromosome counting, however, showed that both the mother suspension and the regenerated sporophytes were diploid, suggesting the involvement of polyteny in the regeneration process.