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NOTE ESTABLISHMENT OF AXENIC CULTURES OF ANABAENA FLOS‐AQUAE AND APHANOTHECE NIDULANS (CYANOBACTERIA) BY LYSOZYME TREATMENT
Author(s) -
Kim JungSeok,
Park YongHa,
Yoon ByungDae,
Oh HeeMock
Publication year - 1999
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1999.3540865.x
Subject(s) - biology , axenic , cyanobacteria , anabaena , lysozyme , microbiology and biotechnology , botany , biochemistry , genetics , bacteria
A new method using lysozyme for the production of axenic cultures of Anabaena flos‐aquae De Brebisson and Aphanothece nidulans P. Richter was developed. Cyanobacterial growth was not inhibited at concentrations up to 1.2 g·L −1 of lysozyme, whereas the growth of heterotrophic bacteria was suppressed. At concentrations up to 0.8 g·L −1 of lysozyme, ampicillin caused a reduction of heterotrophic bacteria. The axenic cultures of these strains were acquired through a simple treatment using 1.0 g·L −1 of lysozyme without ampicillin. These cyanobacteria resisted digestion by lysozyme at our experimental concentrations, whereas bacteria were digested selectively. This method of purification seems to be especially useful with cyanobacterial species that are sensitive to antibiotics or other germicidal agents.