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ACCUMULATION OF SULFURIC ACID IN DICTYOTALES (PHAEOPHYCEAE): TAXONOMIC DISTRIBUTION AND ION CHROMATOGRAPHY OF CELL EXTRACTS
Author(s) -
Sasaki Hideaki,
Kataoka Hironao,
Kamiya Mitsunobu,
Kawai Hiroshi
Publication year - 1999
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1999.3540732.x
Subject(s) - ion chromatography , distilled water , botany , biology , sulfuric acid , algae , seawater , vacuole , inorganic ions , ion , environmental chemistry , chromatography , inorganic chemistry , chemistry , biochemistry , ecology , organic chemistry , cytoplasm
Four species in the order Dictyotales ( Dictyopteris latiuscula (Okamura) Okamura, D. prolifera (Okamura) Okamura, D. repens (Okamura) Børgesen, and Spatoglossum crassum J. Tanaka) were found to be highly acidic as in some species of the order Desmarestiales (Phaeophyceae). The pH within their cells, presumably that of the vacuole, was estimated to be 0.5 to 0.9 by pH measurements of their cell extracts in distilled water. However, other species of these genera ( D. divaricata (Okamura) Okamura, D. undulata Holmes, and S. pacificum Yendo) did not show high acidity. Ion chromatography of the cell extracts showed that those species contained high concentrations of SO within their cells, up to 10 times that in seawater but relatively low Cl − . The sum of cations examined (Na + , NH, K + , Mg 2+ , Ca 2+ ) was significantly lower than that of anions (Cl − , Br − , NO, SO), and the difference is presumed to represent protons (H + ), causing the extremely low cell sap pH. Estimated cellular proton concentrations calculated from the pH data roughly agreed with those calculated from differences between the sum of cations and anions and that of anions. Although certain other, nonacidic, dictyotalean species also contained high concentrations of SO, these species contained high concentrations of Mg 2+ , and the sums of cations and anions were balanced.