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INTRAGENOMIC NR DNA ITS POLYMORPHISM IN THE STEPHANODISCUS NIAGARAE COMPLEX (BACILLARIOPHYCEAE)
Author(s) -
Hartung L. L.,
Goertzen L.R.,
Theriot E.C.
Publication year - 2000
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1999.00001-86.x
Subject(s) - biology , phylogenetic tree , genetics , polymorphism (computer science) , phylogenetics , evolutionary biology , cloning (programming) , genotype , gene , computer science , programming language
Intragenomic nrDNA ITS variability was examined in representative species of the Stephanodiscus niagarae complex. PCR was performed on DNA extracted from monoclonal cultures, and sequence variability determined using standard cloning techniques. Preliminary data from all species reveal intragenomic polymorphism. ITS sequence data is being used to delineate closely related species of algae and polymorphisms are potentially problematic for phylogenetic reconstruction and identity. We are exploring the extent of polymorphism in other freshwater Thalassiorsiraceae, including Stephanodiscus hantzschii fo. tenuis and Cyclostephanos invisitatus.