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A RAPID, HIGHLY EFFICIENT METHOD FOR COLLECTING, FIXING, AND EMBEDDING PLANKTONIC AND OTHER SMALL CELLS FOR ELECTRON MICROSCOPY
Author(s) -
PickettHeaps Jeremy D.
Publication year - 1998
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1998.341088.x
Subject(s) - biology , fixation (population genetics) , dehydration , electron microscope , embedding , filtration (mathematics) , yield (engineering) , microscopy , computational biology , materials science , biochemistry , computer science , optics , artificial intelligence , composite material , physics , mathematics , statistics , gene
A rapid, highly efficient method is described for fixation, dehydration, and embedding of small (e.g. planktonic) cells dispersed in large volumes of culture medium. The entire protocol, based on continuous filtration, can be completed within about an hour, and the yield of cells is very high. Fixation quality has been excellent with several different types of samples.