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DETECTION OF NEW PIGMENTS FROM EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) BY HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHY, LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY, VISIBLE SPECTROSCOPY, AND FAST ATOM BOMBARDMENT MASS SPECTROMETRY
Author(s) -
Garrido José L.,
Zapata Manuel
Publication year - 1998
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1998.340070.x
Subject(s) - emiliania huxleyi , mass spectrometry , chromatography , fast atom bombardment , fucoxanthin , high performance liquid chromatography , pigment , mass spectrum , peridinin , molecular mass , atmospheric pressure chemical ionization , chemistry , analytical chemistry (journal) , chemical ionization , ionization , organic chemistry , phytoplankton , ion , nutrient , enzyme
Pigment extracts from Emiliania huxleyi (Lohm.) Hay et Mohler (strains CCMP 370, CCMP 373, and NIOZ CH 24) were analyzed using high‐performance liquid chromatography (HPLC) on highly efficient monomeric and polymeric octadecylsilica columns using either ammonium acetate or pyridine containing mobile phases. Both systems showed chromatographic profiles with peaks corresponding to pigments of uncertain structure: those of the polar and nonpolar chlorophyll c forms and one peak whose on‐line diode array spectrum resembled that of the fucoxanthin acyloxy derivatives. Liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry gave a molecular mass of 786 units for the unknown carotenoid. The pigments corresponding to each of these fractions were isolated and their visible spectra recorded in various solvents. Samples of the isolated pigments were subjected to analysis by fast atom bombardment mass spectrometry that confirmed a molecular mass of 786 for the unknown carotenoid and gave a mass of 654 units for the polar chlorophyll c 3 , compatible with the monovinylic structure previously suggested. The detection of these new pigments calls for attention on the use of correct methodologies when HPLC pigment signatures are used to study the taxonomic composition of natural phytoplankton populations.