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Abstracts of the 8th Meeting of the Italian Peripheral Nerve Study Group: 31
Author(s) -
Quattrini A,
Biffi A,
Previtali S,
Dina G,
Bordig C,
Naldini L
Publication year - 2003
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1046/j.1529-8027.2003.00031.x
Subject(s) - green fluorescent protein , dorsal root ganglion , sciatic nerve , haematopoiesis , myelin , pathology , stem cell , medicine , bone marrow , transplantation , population , biology , microbiology and biotechnology , anatomy , immunology , central nervous system , dorsum , biochemistry , environmental health , gene
Autologous hematopoietic stem cells (HSC) have been transferred with third generation lentiviral vectors, expressing the green fluorescent protein (GFP), under the human PGK promoter, and transplanted in wild type mice. Transplanted mice have been sacrificed at 6, and 9 months after bone marrow transplantation (BMT) to study the timing and extent of migration of BM derived transduced cells into peripheral nervous system (PNS). Starting from 6 months after BMT cells have been found in the PNS, such as in the acoustic ganglion, the dorsal root ganglions (DRG) and the sciatic nerve. At 9 months from transplant GFP positive cells represented the 50–60% of the F4/80 (a microglial marker) positive population of sciatic nerve sections and the 65–75% of F4/80 + cells of the acoustic ganglion and DRG. At these sites, GFP positive cells were found between nerve fibers and neurons, they expressed F4/80, and were negative for GFAP, neuronal and myelin markers. Autologous BMT coupled with HSC transduction with high efficiency vectors could provide therapeutic benefit to several disorders affecting the PNS such as many lysosomal storage disorders. In these patients clinical evidence confirms the relevance of PNS involvement in the prognosis of the disease.