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Relationship Between Aldose Reductase And Oxidative Stress In Diabetic Peripheral Nerve
Author(s) -
Obrosova Ig,
Stevens Mj,
Fathallah L
Publication year - 2000
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1046/j.1529-8027.2000abstracts-33.x
Subject(s) - aldose reductase , oxidative stress , endocrinology , medicine , sorbinil , malondialdehyde , sorbitol , aldose reductase inhibitor , lipoic acid , polyol pathway , superoxide dismutase , diabetes mellitus , peripheral neuropathy , vitamin c , glutathione , chemistry , antioxidant , biochemistry , enzyme
The relationship between aldose reductase (AR) activity and oxidative stress in tissue sites for diabetic complications remains controversial. We evaluated the ARI sorbinil and three antioxidants, i.e. DL‐α‐lipoic acid (LA), taurine (T) and vitamin E (VE), on indices of oxidative stress and sorbitol pathway intermediates in diabetic peripheral nerve. The groups included control (C) and streptozotocin‐diabetic (D) rats treated with/without: 1.ARI (65 mg/kg, for 2 wks after 4 wks of untreated diabetes); 2.T (1% of the diet); 3.VE (1% of the diet); or 4.LA(100 mg/kg i.p.). T, VE and LA were administered for 6 wks from induction of diabetes. Malondialdehyde plus 4‐hydroxyalkenal levels were increased in D vs C (p < 0.01) and this increase was corrected in D+ARI (p < 0.01 vs D). GSH and ascorbate levels as well as superoxide dismutase activity were decreased in D vs C, and this decrease was completely (GSH) or partially corrected by ARI. Three antioxidants counteracted oxidative stress, but not accumulation of the sorbitol pathway intermediates in the diabetic nerve. In conclusion, increased AR activity contributes to rather than protects from oxidative stress in the diabetic nerve. Oxidative stress is not a cause of sorbitol pathway intermediate accumulation in experimental diabetic neuropathy.