Premium
MACROPHAGE INFILTRATION AND INDUCTION OF P75 NTR AND IL‐1B IN THE NERVE OF DIABETIC RATS
Author(s) -
Conti G.,
De Riz M.,
Scarpini E.,
Bussini S.,
De Pol A.,
Vaccina F.,
Bianchi R.,
Livraghi S.,
Baron P,
Stoll G.,
Scarlato G.
Publication year - 2000
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1046/j.1529-8027.2000.00513-15.x
Subject(s) - infiltration (hvac) , dapi , tunel assay , immunocytochemistry , inflammation , diabetic neuropathy , medicine , apoptosis , pathology , sciatic nerve , regeneration (biology) , nerve fiber , immunohistochemistry , staining , diabetes mellitus , endocrinology , biology , anatomy , microbiology and biotechnology , biochemistry , physics , thermodynamics
Recently, inflammation has been involved in the pathogenesis of diabetic neuropathy, and activated macrophages have been found in the peripheral nervous system of diabetic rats, with a possible role in chemotaxis and regeneration. In this study, we obtained sciatic nerve specimens from diabetic rats at different time points following STZ administration. Macrophages infiltration, IL‐1b and p75NTR induction were analyzed by immunocytochemistry on frozen sections and on teased nerve fibers. Apoptosis was detected on teased nerve fibers by TUNEL and DAPI staining. Cell phenotype was characterized by double‐staining with antibodies specific for Schwann cells and macrophages. The nerves obtained from STZ‐diabetic rats showed macrophages infiltration by day 14 following STZ administration, with complete clearance by day 35. Fifteen percent of these cells were TUNEL positive. IL‐1B induction was concomitant with macrophages infiltration and not detectable by day 35. p75NTR expression began by day 21, peaking by day 35, and dropping to barely detectable levels by day 105. These findings seem to indicate that the concomitance of these processes may be crucial in the regulation of nerve damage and in promoting an attempt of regeneration at the early stages of STZ diabetic neuropathy.