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Effect Of Renal Denervation On Urinary TGF‐β1, Albuminuria And Renal Content Of Glut1 In Stz‐Induced Diabetic Rats
Author(s) -
Schmid H,
Schaan B,
Bertolluci M,
Lacchini S,
Machado U,
Irigoyen Mc
Publication year - 2000
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1046/j.1529-8027.2000.005003187.x
Subject(s) - endocrinology , medicine , diabetic nephropathy , albuminuria , streptozotocin , denervation , diabetes mellitus , glut1 , renal cortex , blood pressure , kidney , urology , glucose transporter , insulin
In long‐term Diabetes Mellitus (DM) the progression of nephropathy has been related to the occurrence of autonomic neuropathy and hypertension. The STZ‐diabetic rat was previously described by us as an animal model that develops albuminuria, as well as increased expression of cortical renal GLUT1 and of Transforming Growth Factor 1 (TGF1) and increased urinary excretion of TGF1. The aim of this study was to evaluate the effect of renal denervation (RD) upon urinary TGF‐β1 (UTGF‐β1) and cortical renal GLUT1 protein levels, relating these parameters to blood pressure (BP) levels and metabolic control. Streptozotocin injected rats (group D; n=13) and control animals (group C; n=13) were compared for their urinary albumin (UA), UTGF‐1, BP and for their renal cortical and medullar GLUT1 protein abundance 45 days after the beginning of hyperglycemia. The animals were submitted to surgical RD or sham procedure within 30 days of STZ injection, and evaluations were done 15 days after the surgery. The effects of RD were confirmed by intra‐renal decrease of norepinephrine levels. Results showed that RD determined no changes in UA or UTGF‐β1, in both D and C rats. Blood pressure levels were similar between D and C and did not change as an effect of denervation. These results are shown in the table. In conclusion, in the STZ‐rat with albuminuria complicating diabetes, RD produces a normalization of previously elevated cortical GLUT1 protein and no effect upon UTGF ‐ β1 and UA, suggesting that these animals (with no hypertension) are protected from the deleterious effect of kidney denervation.