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Low Concentration of DL‐2‐Amino‐5‐phosphonovalerate Induces Epileptiform Activity in Guinea Pig Hippocampal Slices
Author(s) -
Gorji Ali,
Speckmann ErwinJosef
Publication year - 2001
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1046/j.1528-1157.2001.01301.x
Subject(s) - nmda receptor , 2 amino 5 phosphonovalerate , hippocampal formation , chemistry , neurotoxicity , phencyclidine , guinea pig , convulsion , excitatory postsynaptic potential , pharmacology , electrophysiology , epilepsy , receptor , medicine , neuroscience , biology , toxicity , biochemistry , excitatory amino acid antagonists , organic chemistry
Summary: Purpose: The specific mechanisms by which low concentrations of cyclosporine induce seizures and low concentrations of phencyclidine provoke behavioral excitation remain to be elucidated. Both compounds block N ‐methyl‐ d ‐aspartate (NMDA) receptors. The aim of this study was to determine if low concentrations of the NMDA‐receptor blockers increase the seizure susceptibility. Methods: Guinea pig hippocampal slices were exposed to artificial cerebrospinal fluid containing the NMDA blocker dl ‐2‐amino‐5‐phosphono‐valerate (APV; 0.1–10 μ M ). Extracellular field potentials were recorded from CA1 and CA3 regions. Results: Low concentrations of APV induced epileptiform burst discharges (0.1–0.25 μ M ), whereas higher doses failed to decrease the seizure threshold (1–10 μ M ). Conclusions: The results indicate that the excitatory effect of low concentrations NMDA blockers may play a role in the neurotoxicity of aforementioned substances.