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Exclusion of 5‐HT 2A and 5‐HT 2C Receptor Genes as Candidate Genes for Migraine
Author(s) -
Buchwalder Anne,
Welch Susan K.,
Peroutka Stephen J.
Publication year - 1996
Publication title -
headache: the journal of head and face pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.14
H-Index - 119
eISSN - 1526-4610
pISSN - 0017-8748
DOI - 10.1046/j.1526-4610.1996.3604254.x
Subject(s) - migraine , genetics , locus (genetics) , biology , candidate gene , gene , genetic linkage , receptor , family aggregation , medicine , disease
Several lines of investigation suggest that the serotonergic system may be involved in the pathogenesis of migraine. In particular, drugs which block 5‐HT 2 receptor subtypes appear to be effective migraine prophylactic agents. Therefore, chromosomal DNA regions overlapping the 5‐HT 2A (13q14‐q22) and 5‐HT 2c (Xq22‐25) receptor loci were analyzed for possible linkage to the clinical diagnosis of migraine. No evidence for linkage to either chromosomal region was found, although a small subset of migrainous families showed positive likelihood of odds (LOD) scores. However, a homogeneity (HOMOG) analysis provided no statistical evidence for locus heterogeneity. The coding region of the 5‐HT 2A and 5‐HT 2c receptor genes was also analyzed in migraine patients and unaffected controls using polmerase chain reaction and direct sequencing. No mutations were found in the deduced amino acid sequence of either receptor in the sample of migraineurs tested. These results indicate that DNA‐based mutations in the 5‐HT 2A and 5‐HT 2c receptors are not generally involved in the pathogenesis of migraine.