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Pyruvate Preserves Neutrophilic Superoxide Production in Acidic, High Glucose‐Enriched Peritoneal Dialysis Solutions
Author(s) -
Wu Yi Tai,
Wu Zhao Long,
Jiang Xiao Feng,
Zhou Fang Qiang
Publication year - 2003
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2003.69962.x
Subject(s) - superoxide , chemistry , peritoneal dialysis , biochemistry , medicine , enzyme
Aim: To investigate effects of pyruvate (Py)‐based peritoneal dialysis solutions (P‐PDS) on neutrophilic superoxide (O 2 − ) production against high glucose (HG) concentrations at acidic or physiologic pH value, and explore potential mechanisms. Methods: Human neutrophils were incubated with both dl ‐lactate (La, 40 mM)‐based PDS (L‐PDS) and equimolar P‐PDS at various pH and HG levels, respectively. Hanks’ balanced salt solution (HBSS) served as controls. O 2 − generation was determined by the reduction of ferricytochrome c. Results: Acidic pH and high La induced acute and substantial inhibitions of O 2 − production. HG in both PDS and HBSS resulted in a suppression of O 2 − in a dose‐dependent manner. P‐PDS generated near twofold O 2 − formation of L‐PDS counterparts at various pH and HG levels. P‐PDS with HG produced significantly more O 2 − than Py‐free HBSS counterparts. Conclusions: Py in PDS effectively protected neutrophils from HG‐induced inhibition of O 2 − production, even at a physiological pH. The Py cytoprotection may be associated with the preservation of carbohydrate metabolic pathways in addition to its alkalization.