Premium
Release of (1→3)‐β‐D‐Glucan from Depth‐type Membrane Filters and Their In Vitro Effects on Proinflammatory Cytokine Production
Author(s) -
Ohata Atsushi,
Usami Makoto,
Horiuchi Takashi,
Nagasawa Koichi,
Kinoshita Keiko
Publication year - 2003
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2003.07137.x
Subject(s) - proinflammatory cytokine , cytokine , tumor necrosis factor alpha , lipopolysaccharide , chemistry , macrophage , in vitro , membrane filter , immunology , biochemistry , biology , inflammation , membrane
To clarify the origin of (1→3)‐β‐D‐glucan in blood products and assess the biological activity of filter extracts, we evaluated (1→3)‐β‐D‐glucan extraction from depth filters used to process blood products and their in vitro effects on proinflammatory cytokine production from macrophages. Cellulose or nylon filters were analyzed for (1→3)‐β‐D‐glucan using the Fungitec G test. To evaluate the biological activity of the filter extracts, Mono Mac 6 cells (a human macrophage cell line) were cultured with filter extracts with or without lipopolysaccharide, and tumor necrosis factor‐alpha (TNF‐α) and interleukin‐1 beta (IL‐1β) concentrations in the culture media were measured. (1→3)‐β‐D‐Glucan was released from seven cellulose filters but the nylon filter level was undetectable. Proinflammatory cytokine production ranged from 74.3% to 119.0% of the control for TNF‐α and 81.2% to 115.9% for IL‐1β. TNF‐α and IL‐1β levels were low without lipopolysaccharide. The data indicate that (1→3)‐β‐D‐glucan in blood products is contaminated with the depth filters and that these filter extracts modulate proinflammatory cytokine production from macrophages.