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Activated Leukocytes Adsorbed on the Surface of an Extracorporeal Circuit
Author(s) -
Tabuchi Noriyuki,
Shibamiya Aya,
Koyama Takatoshi,
Fukuda Tetsuya,
Oeveren Willem van,
Sunamori Makoto
Publication year - 2003
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2003.07050.x
Subject(s) - extracorporeal circulation , biocompatibility , elution , cardiopulmonary bypass , adsorption , chemistry , extracorporeal , chromatography , medicine , surgery , organic chemistry
Biocompatibility of extracorporeal circuits has mostly been investigated by sampling blood in circulation. However, a proportion of activated leukocytes leave circulation by sticking to the circuits, and might affect the circuit biocompatibility. To reveal these characteristics, we eluted the adsorbed leukocytes from circuits used for 6 patients by washing with ethylenediaminetetraacetic acid (EDTA)‐HEPES buffer. 1.3 × 10 9 leukocytes, representing 5 to 10% of the circulating leukocytes, were collected. Most were neutrophils expressing CD11b antigens as high as those in circulation during cardiopulmonary bypass. Adsorbed monocytes expressed tissue factor higher than those in circulation ( P < 0.05). An elution procedure by EDTA itself induced only a minimal activation of the leukocytes. These results indicate that extracorporeal circuits adsorb a large number of activated neutrophils and a small number of highly thrombogenic monocytes during their use. Although our data is limited, this elution procedure appears useful to investigate the biocompatibility of extracorporeal circuits.