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Functional Analysis of the Tissue‐Engineered Stomach Wall
Author(s) -
Hori Yoshio,
Nakamura Tatsuo,
Kimura Dai,
Kaino Kenji,
Kurokawa Yoshimochi,
Satomi Susumu,
Shimizu Yasuhiko
Publication year - 2002
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2002.07006.x
Subject(s) - stomach , tissue engineering , anatomy , regeneration (biology) , beagle , scaffold , biomedical engineering , chemistry , medicine , biology , microbiology and biotechnology
We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissue‐engineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 × 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissue‐engineered gastric tissue. However, acetylcholine‐induced contraction was not observed in the tissue‐engineered stomach wall. Although there is still room for improvement, the tissue‐engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.