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Histological and Immunohistological Study of Cryopreserved Aortic Valve Grafts: The Possibility of a Clinical Application for Cryopreserved Aortic Valve Xenograft
Author(s) -
Yokose Shogo,
Fukunaga Shuji,
Tayama Eiki,
Kato Seiya,
Aoyagi Shigeaki
Publication year - 2002
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2002.06974.x
Subject(s) - cryopreservation , medicine , aorta , aortic valve , transplantation , abdominal aorta , immunohistochemistry , surgery , pathology , biology , embryo , microbiology and biotechnology
The influence of preservation techniques on xenogeneic immune responses was examined to investigate the possibility of their clinical use. Mongrel dogs and pigs were used as allogeneic and discordant xenogeneic donors of aortic valve grafts, respectively. The allografts and xenografts were implanted in the abdominal aorta of mongrel dogs after cryopreservation or after fresh preservation. Allografts and xenografts were explanted at Days 7 and 42 for histological and immunohistochemical examination. In the fresh preserved allografts and the fresh preserved xenografts, the media of aortic wall grafts were significantly acellular at 7 days. Significant aortic medial destruction and neointimal proliferation with significant destruction of the valve leaflets was observed at 42 days. However, no differences were found among the preservation techniques in the xenogeneic grafts. To alleviate the lack of homografts, approaches such as the tissue‐engineered graft may become an alternative in patients who are acceptable candidates.