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Effect of Different Solvents and Crosslinkers on Cytocompatibility of Type II Collagen Scaffolds for Chondrocyte Seeding
Author(s) -
Tsai ChingLin,
Hsu Shanhui,
Cheng WenLing
Publication year - 2002
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2002.06732.x
Subject(s) - carbodiimide , chondrocyte , chemistry , adhesion , acetic acid , type ii collagen , cartilage , tissue engineering , matrix (chemical analysis) , type i collagen , polymer chemistry , chemical engineering , biomedical engineering , biophysics , in vitro , biochemistry , chromatography , anatomy , organic chemistry , medicine , biology , engineering , pathology
Type II collagen extracted from porcine costal cartilage was evaluated as scaffolds for cartilage tissue engineering. Chemical crosslinkers were employed to improve the mechanical properties and the resistance toward degradation. Films and porous scaffolds were prepared from collagen solutions dissolved in 3% acetic acid (designated A) or in deionized water (designated W) and crosslinked by an epoxy (designated E) or by a carbodiimide (designated C). Immortalized rat chondrocytes and rabbit chondrocytes were used to assess cytocompatibility of crosslinked collagen matrices. Cell adhesion rate onto the films made by different preparations ranked in the order of WE ≥ WC > AC ≥ AE. Cell proliferation ranked in the order of AC > WC > AE ≥ WE. Cells maintained round morphology only on AC and WC films. In 3‐D seeding, AC scaffolds also were found to be the most cytocompatible. WC scaffolds, however, had better dimensional stability. It was concluded that Type II collagen scaffolds, when prepared by using deionized water as the solvent and carbodiimide as the crosslinker, could promote chondrocyte growth and matrix production.